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Almost every main quest mission gives a karma boost at the end unless there are multiple outcomes, then you just have to NOT do the evil ones. Also, outside of Megaton and Rivet City is a water beggar, give him some purified water to boost karma, you can do this as many times as you want. If you have the house in Megaton or the Tenpenny Suite you can have your Mr. Handy give you 5 bottles of water every 3 days.