Physically yes, spiritually no. He was physically killed when sealing Kyuubi into his son Naruto. However, he recently appeared in the seal that binds the Kyuubi to prevent Naruto from releasing it. Sort of a fail safe measure. He appeared just long enough to briefly talk to Naruto about the situation and inform him that Madara Uchiha is the guy behind the curtain. He also restored the seal to suppress the Kyuubi's chakra during Naruto's fight with Deva Path Pein.