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12 lacewing flies, 2 ounces crude Antimony, 4 leeches, 16 scruples of flaxweed, 3 drachms of Sal Ammoniac (crystalized ammonia, made liquid), 15 knotgrass, 1 pinch of powdered horn of bicorn (lunar extracted), shredded skin Boomslang, Filings and rasplings of Salpeter, Mercury and Mars, DNA of the transfigured-to-be.

Taken from the Polyjuice Potion page at the Harry Potter Wiki site:

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